To investigate whether FgGMTV1 infection enhances the methylation level of the integrated pRep(500)-GFP, we introduced the infectious FgGMTV1 clone into PH-1::pRep(500)-GFP and dim2::pRep(500)-GFP strains through PEG-mediated protoplast transfection (Supplementary Fig. S5A). Here, TXNL4B is linked to infection.