Additionally, aberrant methylation patterns in the AKR1B1, RASGRF2, CRMP1, BNIP3, GSTP1, HOXA5, and PAX6 genes have been observed in estrogen receptor (ER)-positive and HER2-negative breast cancer with axillary lymph node metastasis (ALNM), suggesting their potential as therapeutic targets [30]. This evidence concerns the gene ESR1 and breast cancer.