To determine whether ZBTB7A upregulation impacts MDD-specific OCR gene targets in human astrocytes, we used a lentivirus to overexpress (OE) ZBTB7A in human primary cortical astrocytes, which resulted in a robust induction of MDD-specific OCR target genes, as well as other key MDD DEGs within the NF-kB pathway (Figure S2L–O). Here, NFKB1 is linked to major depressive disorder.