Using these 20 genes, further gene correlation analysis via Gene Expression Profiling Interactive Analysis (GEPIA) confirms a negative correlation between P2RX4 and IGFN1 (Supplementary Fig. 3a) and a positive correlation between P2RX4 and PCDHB7, SERPINH1, and CACNB4 (Supplementary Fig. 3b-d) in TCGA prostate adenocarcinoma clinical database. The gene discussed is CACNB4; the disease is prostate adenocarcinoma.