Given that the RAS/MAPK and AKT/mTOR/c-Myc pathways are frequently activated in almost 50% of HCC patients [46], we performed hydrodynamic tail vein transfection of N-Ras and c-Myc proto-oncogene activation forms, which were stably integrated into the hepatocyte genome after transient expression of Sleeping Beauty transposase (SBT), for HCC induction. This evidence concerns the gene NRAS and hepatocellular carcinoma.