MAPT and Alzheimer disease: Under the conditions optimized for the differentiation of conformers of misfolded tau, the hydroxylation rate of tau441 monomers was 30–200-fold faster than that of insoluble aggregates of AD brain-derived tau, and all epitopes were fully hydroxylated in the first two minutes (green line in S1B, S1C, S1D, S1E, and S1F Fig).