To investigate the tau propagation tendency of distinct tau conformers, we inoculated wild-type primary mouse neurons with structurally characterized Sarkosyl-insoluble tau isolates from the frontal cortex of six AD cases and monitored the accumulation of de novo-induced tau aggregates using confocal microscopy (Fig 3D) and CDI (Fig 3E) [27]. The gene discussed is MAPT; the disease is Alzheimer disease.