The immunostaining of RA-differentiated SH-SY5Y cultures with tau R1 MTBD-specific (clone 16040D, 269–281 aa) and hyperphosphorylated tau-specific AT8 antibody revealed variability among individual AD brain-derived tau seeds to trigger tau misfolding and pathological phosphorylation with a delay in the deposition of tau aggregates phosphorylated at Ser202 and Thr205 (Figs 5A, 5B, 5E, 5F, and S6C). The gene discussed is MAPT; the disease is Alzheimer disease.