Since our SCFM2-Scnn1b-Tg mouse model utilizes SCFM2, and SCFM2 was shown to induce P. aeruginosa transcriptional profiles similar to those in human CF sputum in normal mice (37), we are confident that this SCFM2-Scnn1b-Tg mouse model will be a valuable tool for investigating potential antimicrobials and the evolution of microbes during chronic infection. Here, SCNN1B is linked to cystic fibrosis.