SCNN1B and cystic fibrosis: Here, we combined these alternative models and established a chronic P. aeruginosa lung infection model using SCFM2 agar beads and Scnn1b-Tg mice (SCFM2-Scnn1b-Tg) to recapitulate nutrient and mucus characteristics of the human CF lung environment and test the effects of chronic infections on bacterial burden, lung function, and the immune response.