To investigate the expression levels of NKG2DLs in human ESCC, we conducted flow cytometry analyses on five ESCC cell lines utilizing antibodies recognizing MICA, MICB, ULBP-1, ULBP-2/5/6, ULBP-3, or ULBP-4. This evidence concerns the gene ULBP1 and esophageal squamous cell carcinoma.