Focusing on genes differentially expressed in the T cell subsets, they found an overexpression of set of genes that control for cellular cytotoxicity, including GNLY, GZMA, GZMB, GZMH, GZMK, PRF1, CTSW, KLRB1, KLRD1, and NKG7. ALS patients exhibited greater levels of TCR expansions in cytotoxic CD4+ T cells as well as in CD4+ and CD8+ T cells with an activated phenotype (CCR7− CCL5+), compared with controls. This evidence concerns the gene CD4 and amyotrophic lateral sclerosis.