In order to further investigate the effect of cyclomorusin and cis-platinum on the apoptosis of lung cancer cells, Hoechst 33342 staining and Annexin V/Propidium Iodide (AnnexinV/PI) flow-cytometry assay were applied to measure cell apoptosis after treatment with cyclomorusin and cis-platinum. The gene discussed is ANXA5; the disease is lung cancer.