In an in vitro co-culture model of RBE4 endothelial cells with U87 glioma cells, the levels of p-JNK and p-c-Jun were increased in RBE4 cells, while the expression of claudin-5 and ZO-1 was decreased compared with RBE4 cells cultured without U87 [23]. This evidence concerns the gene TJP1 and central nervous system cancer.