In light of the central role of hENT-1 in regulating the cellular uptake of nucleosides and nucleoside-analogue drugs, in the present study we investigated the possible link between p53 status and hENT-1 regulation in iCCA patients and cell line models; the predictive role of p53 status in the response to adjuvant gemcitabine of iCCA patients was also investigated. This evidence concerns the gene TP53 and infantile convulsions and choreoathetosis.