Next, to elucidate whether PBX3 regulation of HMGCR is involved in its role as a positive regulator of cholesterol metabolism, we constructed an HMGCR overexpression vector (Figure S3) and overexpressed HMGCR in PBX3-knocked down HCC-LM3 cells (Figure 3A). The gene discussed is PBX3; the disease is hepatocellular carcinoma.