To determine the role of DUX4 expression in the higher m6A methylation, we treated the FSHD myoblasts with AON-targeting DUX4 for 24 h, and we found that the m6A percentage was significantly reduced in the FSHD myoblasts to the level of the UASbs (Figure 3). This evidence concerns the gene DUX4 and facioscapulohumeral muscular dystrophy.