Our aim was to evaluate the diagnostic performance of ITSS for tumor grade prediction, as well as to assess the correlation of ITSS with key radiological, histopathological, and molecular markers, including rCBV, tumor volume, mitotic activity, Ki-67 index, necrosis, microvascular proliferation, IDH mutation, 1p/19q co-deletion, CDKN2A/B deletion, and p53 expression. Here, TP53 is linked to neoplasm.