CD4 and neoplasm: To examine the impact of M3258 treatment on the TME, we performed sc-RNAseq analysis of the SUM-149 PT xenograft tumors from the experiment depicted in Figure 4A. On the basis of DGE profiles, the tumors were divided into nine clusters, including one doublet cell population and eight singlet cell populations representing epithelial cells (tumor cells), CD4+ T cells, CD8+ T cells, Tregs, M1 macrophages, M2 macrophages, dendritic cells, and mast cells (Figure 4B).