When KRAS G12C was not detected in DNA isolated from organoid cultures L8 and L11 by Sanger Sequencing requiring 25–30% tumor cells, a real-time PCR assay requiring only 10% tumor cells was applied: The Thyroid Cancer Mutation Detection Kit (EntroGen) on ABI Applied Biosystems Fast 7500 Real-Time PCR system (ThermoFisher). This evidence concerns the gene KRAS and thyroid cancer.