On the subcellular level, a focused ultrasound (FUS) that has an amplitude of 46 MHz (12 Vp–p), pulse repetition of 1 kHz frequency, and duty of 5% cycle, stimulates ICWs in PC-3 prostate cancer cells via mechanosensitive pannexin-1 (PANX1) channels in the ER without cytoskeletal dependence [88]. This evidence concerns the gene PANX1 and Familial prostate cancer.