TOP2A and cancer: However, rather than exhibiting classic features of stem-like cells, sub-cluster 5 was characterized by unique gene expression profiles, including genes like TOP2A, PBK, CCNB2, DLGAP5, CENPA, HMMR, MKI67, and BIRC5, all of which are known to be associated with cell proliferation and cancer progression [31,32,33,34,35,36,37,38] (Figure 1H).