In other cases, we target specific exons where the main hotspots of that gene are located, such as with the KIT gene, in which, in routine diagnostics, we study exon 17 (p.D816V) and exon 8 because they contain variants that provide a definite diagnosis of mastocytosis [1,2] and are associated with an unfavorable prognosis in AML patients [3,4]. This evidence concerns the gene KIT and acute myeloid leukemia.