The data from our immunofluorescence and proximity labeling studies of STX17 suggest that the bacterial vacuoles formed during the initial stages of infection are derived from hybrid phagosomes (which have early endosome markers like Rab5, EEA1, and ATG16L) and STX17+ pre-autophagosomes originating from the ER. This evidence concerns the gene ATG16L1 and infection.