Given the elevated pro-inflammatory response and reduced bacterial load in Nedd9-/- mBMDMs in vitro, we investigated the impact of Nedd9 loss on bacterial clearance in vivo by challenging Nedd9wt/wt and Nedd9-/- mice intraperitoneally with ST. In line with the in vitro data, Nedd9 loss significantly enhanced the pro-inflammatory responses upon ST infection, as demonstrated by elevated cytokine IL-6 in spleens of ST-infected Nedd9-/- compared to Nedd9wt/wt mice (Fig. 4A) while loss of Nedd9 did not affect body or spleen weight upon infection (Supplementary Fig. S3A, B). This evidence concerns the gene NEDD9 and infection.