To further identify the phagocytosis-related key molecules and pathways, PAMs were transfected with siRNAs targeting TLR2, CD14, CD48, CD64, or SDC4 for 24 h, respectively, followed by infection with ASFV for another 24 h, and then their phagocytosis functions were evaluated by adding a low dose of E. coli-EGFP (MOI 5). This evidence concerns the gene SDC4 and infection.