Since a poly-Glu is a known substrate for PSMA,the MTX-based peptidebioconjugates were evaluated for their in vitro stabilityand selective toxicity using PSMA-expressing, PSMA­(+) LNCaP PCa cellsand non-PSMA expressing, PSMA(−) TSU, human bladder cancercells. The gene discussed is FOLH1; the disease is urogenital neoplasm.