We developed a highly sensitive sandwichimmunoassay that integratesfluorescent silica nanoparticles and bioorthogonal chemistries forthe ultrasensitive detection of host biomarkers (MCP-1 and IP-10)and a viral biomarker (NS1) for early dengue diagnosis and monitoringdisease progression. The gene discussed is CXCL10; the disease is dengue disease.