Quantitative real-time PCR (real-time qPCR) was performed to analyze the mRNA levels of PAI-1 and CHRNA1 in sweat gland tissues from healthy controls (HCs, n = 60), primary axillary hyperhidrosis (PAH, n = 68), primary craniofacial hyperhidrosis (PCH, n = 68), and primary palmar hyperhidrosis (PPH, n = 68) (Figure 1). The gene discussed is CHRNA1; the disease is pulmonary arterial hypertension.