We used whole exon sequencing to sequence paired OC tissue samples from 32 ctDNA mutation-positive patients and found TP53, PIK3CA, and APC were also the most frequently mutated genes in the tumor biopsies, and the concordance rate between ctDNA and tumor tissues was 68.8% (Table S4), which was similar to that reported in previous studies.32 This evidence concerns the gene PIK3CA and neoplasm.