LFBK cells stimulated with transfected poly(dA:dT) (10 μg/mL) to detect IFN-β, IFN-α, IRF7, IRF3, PKR, MX1, ISG56, and ISG15 at 2, 24, and 48 h infection times using qRT-PCR was investigated (Figure 2A). This evidence concerns the gene ISG15 and infection.