These results coincide with a previous report using trophozoites of Acanthamoeba castellanii, in which a decrease of 90% in adhesion of trophozoites to fibroblasts was reported by Rodríguez et al.25In 2005, Garate et al. have also demonstrated that polyclonal anti-mannose-binding protein antibodies, prepared in chickens, were potent inhibitors of Acanthamoeba adhesion to epithelial cells, after evidencing the key role of this protein in the pathogenesis of Acanthamoeba infection by mediating host-parasite interactions.36 Here, MBL2 is linked to Acanthamoeba infectious disease.