Analyzing PD‐1 and TIGIT expression on CD4+ T cells on samples from 5‐ALA assisted surgery, we observed a higher percentage of PD‐1+ T cells in all analyzed different tumor areas than in PB, whereas no difference was observed for TIGIT and for both ICs expression among the three different GBM layers (Figure 2J,L). Here, CD4 is linked to neoplasm.