TRPC3 and Alzheimer disease: Although the quantification of the upregulated TRPC3 immuno-DAB signals using two different approaches (Figure 1F) reveals a slightly larger increase in TRPC3 in pyramidal neurons than the total cells in the AD versus NC samples, the increase in the TRPC3 expression in the latter case (i.e., mixed brain cells) is equally significant (p < 0.01).