The specifics of ZBTB20 do not provide direct evidence of its transcriptional regulation of the two key transcription factors, AFP and FOXO1, in HCC, although ZF domains are potential candidates for these interactions.23,33 Notably, the arrangement of ZF domains shows that the first four ZF domains, ZBTB20(ZF1-4), are linked to the 5th ZF domain by 25 amino acids (Fig. 1a). Here, ZBTB20 is linked to hepatocellular carcinoma.