ZBTB20 inhibits HCC by inhibiting AFP and induces HCC by inhibiting FOXO1.23,33 In this study, we examined the Kds, specific sequences, and metal interferences to scrutinize these binding events through overexpressed and purified Zn2+–ZBTB20(ZF1-4) and Zn2+–ZBTB20(ZF1-5). This evidence concerns the gene AFP and hepatocellular carcinoma.