Therefore, given the functional importance of CDK6 in regulating the growth of SHH-medulloblastoma cells [28], we transfected SHH-DAOY cells with miR-449a mimic or non-functional (negative control miRNA mimic) miRNAs, and measured CDK6 protein expression 72 h post-treatment. The gene discussed is CDK6; the disease is medulloblastoma.