In this study, we found that VILIP3 expression was downregulated in the hippocampus of 5×FAD mice and in Aβ1−42-interfered SH-SY5Y cells, which is consistent with previous reports of reduced VILIP3 expression in neocortical areas of AD patients in clinical studies (Braunewell et al. 2001), suggesting that abnormal VILIP3 expression may be a general feature of AD pathology. The gene discussed is HPCAL1; the disease is Alzheimer disease.