In contrast, Treg expansion in the IL-2/JES6-1 group significantly improved cardiac function, with higher LVEF and LVFS, and lower LVDV and LVSV (P < 0.05).Histological analysis (Fig. 2B and C) revealed a significantly larger myocardial infarct area in the Ctrl and IgG groups compared to the Sham group (P < 0.05), with a further increase in the anti-CD25 group. This evidence concerns the gene IL2 and myocardial infarction.