However, immunofluorescence staining (Fig. 2L-N) revealed no significant differences in the number of CD31-positive capillaries or α-SMA-positive small arteriolar vessels between the Treg and Ctrl groups, as well as between the anti-CD25 and anti-CD25 + Tregs groups (P > 0.05).These results indicate that while Treg transfer significantly reduces infarct size and improves cardiac function after AMI, it does not have a significant effect on post-infarction neovascularization, similar to the effects of endogenous Treg amplification. Here, ACTA1 is linked to infarction.