The percentage of TNF+ (Fig. 3A,C) and IFN-γ +TNF+ (Fig. 3A,E) Irf5−/− p14 cells was also significantly lower than the control group at d30 and d60 p.i. Interestingly, mice that received Irf5−/− p14 cells showed reduced frequencies of IL-2-producing cells over the whole course of infection compared with those that were adoptively transferred with WT p14 (Fig. 3A,D, upper panel). This evidence concerns the gene TNF and infection.