Prior studies reported that sid-3/TNK2, which encodes a cytoplasmic tyrosine kinase implicated in RNA import and endosomal trafficking, is critical for Orsay virus infection.46,63,64 Eliminating SID-3 enhances resistance to viral infection in worms, likely due to a defect in viral entry.46 Interestingly, we found that knocking out sid-3 leads to a modest but statistically significant drop in transgene expression (q = 0.0004; Figure 1F). This evidence concerns the gene TNK2 and viral infectious disease.