To study the role of DAF on neutrophil activation and AKI severity, we administered AA (5 mg/kg, Sigma-Aldrich, 102501333) i.p. every other day for 5 days (day 1, 3 and 5) to 8-week-old C57BL/6J (B6, H-2b) male mice that were euthanize at 14 days after the first injection. Here, CD55 is linked to acute kidney injury.