The goal of this work was to study the specific mechanism by which SENP3 regulates downstream genes through deSUMOylation and thus affects the progression of osteoporosis.<h4>Methods</h4>Osteogenic differentiation was evaluated through osteogenic marker genes, mineralization, and ALP activity, which were detected by qPCR, western blot, and ALP staining assays. The gene discussed is SENP3; the disease is osteoporosis.