To further evaluate the effects of inhibiting Nampt‐mediated NAD+ metabolism in the MC38 and CT26 CRC cell lines, different concentrations of FK866, an inhibitor of Nampt, were used on MC38 and CT26 cells for 24, 48, and 72 h; then, cell viability was measured by a CCK8 assay. This evidence concerns the gene NAMPT and colorectal carcinoma.