Intriguingly, mass cytometry time‐of‐flight (CyTOF) analysis indicates that the combined treatment with FK866 and C188‐9 exerts antitumor effects by increasing the infiltration of CD8+ T cells and neutrophils into the tumor, as well as enhancing the expression of immune‐regulatory molecules, including IFN‐γ, IL‐10, and perforin. This evidence concerns the gene PRF1 and neoplasm.