HOMER1 and amyloidosis: To quantify synapse loss, we labeled presynaptic and postsynaptic compartments in the dentate gyrus of 6-mo-old App/Ps1-/- and App/Ps1+/- littermates by detecting synaptic vesicle protein 2 (SV2) and Homer1, respectively, and selected multiple fields of interest per animal for imaging analysis while avoiding regions adjacent to amyloid deposits (Figure 7—figure supplement 2).