When QUE acts on the TME of melanoma, it not only inhibits the proliferation and migration of B16 cells but also increases the number of M1 macrophages and CD8+ T cells by inhibiting PDK1/CD47 signals and promoting the secretion of IL-2 and IFN-γ by CD8+ T cells, thereby inhibiting the growth of melanoma (146). The gene discussed is PDK1; the disease is melanoma.