DEFA3 and tuberculosis: Validation in an independent dataset comprising 90 TB patients and 20 healthy controls confirmed the robustness of the 10-gene signature (BATF2, FAM20A, FBLN2, AK5, VAMP5, MMP8, KLHDC8B, LINC00402, DEFA3, and GBP6), achieving high area under the curve (AUC) values in both receiver operating characteristic (ROC) and precision–recall analyses.