Validation in an independent dataset comprising 90 TB patients and 20 healthy controls confirmed the robustness of the 10-gene signature (BATF2, FAM20A, FBLN2, AK5, VAMP5, MMP8, KLHDC8B, LINC00402, DEFA3, and GBP6), achieving high area under the curve (AUC) values in both receiver operating characteristic (ROC) and precision–recall analyses. Here, FBLN2 is linked to tuberculosis.