To understand the molecular mechanism underlying the influence of Lr EVs on calcium handling in breast cancer cells, we performed a real-time RT-PCR study of several genes, including PMCA2, calcium channel ORAI-1, calcium sensor STIM1, and apoptotic marker BCL-2, to determine whether their expression varied upon exposure to bacterial EVs (Figure 7G). The gene discussed is STIM1; the disease is breast cancer.