However, we found that TSH synergistically enhanced TGF-β-induced EMT in RET mutated thyroid cell lines (TPC-1) on both mRNA and protein levels, but only synergistically enhanced TGF-β-induced EMT in E-cadherin and N-cadherin, as shown in Figs. 6 and 7. The gene discussed is RET; the disease is thyroid gland disorder.