As increases in dietary ω-3 PUFAs have previously been linked with a reduction in cancer development through lowering of AA levels within tissue phospholipid membranes and a decrease in the production of proinflammatory AA-derived prostaglandins (PG) (43), our results suggest that increased tumor burden in mice fed a high ω-6:ω-3 ratio diet may be driven by increased production of proinflammatory AA-derived oxylipins produced by LOX, due to reduced availability of ω-3-derived EPA and DHA substrate. This evidence concerns the gene LOX and cancer.