To model the vascular effects of overnutrition in WD-fed mice, we cultured human dermal microvascular endothelial cells (HDMECs) under diet-mimicking conditions for 6 days, using a mixture of 100 μM palmitate, 25 mM d-glucose, and 1 μM insulin (Pal/Glu/Ins). The gene discussed is INS; the disease is overnutrition.