BACE1 and neurodegenerative disease: To determine the potential mechanism of BACE1-AS, the location of BACE1-AS in HCT-8 cells was identified by using the FISH assay, and the results indicated that BACE1-AS was majorly located outside of the nucleus of HCT-8 cells (Fig. 3A), reflecting that BACE1-AS likely functioned by sponging miRNAs, which has been reported in human diseases, particularly neurodegenerative diseases and cancers (35, –, 39).