In the OFT (Figure 2—figure supplement 1A–D), Trio variant mice did not differ significantly from WT in total distance traveled or mean speed; however, +/K1431M females spent a greater amount of time in the outer and corner zones of the OFT compared to WT (Figure 2—figure supplement 1D, right), suggesting increased anxiety-like behavior in +/K1431M female mice. Here, TRIO is linked to Anxiety.