MGMT and glioma: In our single-center sample of patients newly diagnosed with grade 2–3 glioma, our main findings showed that the absolute prevalence of fluorescence positivity was 36.6 % for 5-ALA and 16 % for FS, with an association with the presence of contrast enhancement foci on MRI, but without an association with histological grade, IDH mutation, or MGMT methylation.