In our single-center sample of patients newly diagnosed with grade 2–3 glioma, our main findings showed that the absolute prevalence of fluorescence positivity was 36.6 % for 5-ALA and 16 % for FS, with an association with the presence of contrast enhancement foci on MRI, but without an association with histological grade, IDH mutation, or MGMT methylation. Here, MGMT is linked to central nervous system cancer.